Molecular test technology amplifies target DNA/RNA by over a billion times providing sensitive and reproducible results.
Seegene has made important contributions to the field of multiplex PCR testing with the development of DPO™, TOCE™, and MuDT™ technologies. Employed across our various products, these innovations make it possible to detect from among multiple targets in a single PCR channel, increasing the total number of candidate targets in a single PCR reaction, compared to standard practice.
DPO™ are primers made of two portions that work together to ensure highly specific hybridization, minimizing dimer formation. The DPO™ primers system is a highly accurate, rapid, and cost-effective method of primer binding. It allows for the detection of many targets in a single PCR reaction and is well-suited for high multiplex real-time PCR technology as it can overcome the limitations of conventional multiplex assays that include low plexing (4-6 targets) per reaction and reductions in specificity due to primer-dimer formation.
- Enables highly multiplex chemistries by minimizing non-specific amplification
- Maximizes specificity and sensitivity
- Prevents primer competition and non-target amplification where primer might bind to itself (hairpins) or to other primers (dimers).
- Withstands a wide range of annealing temperatures
- Accurate, rapid, and cost-effective method of primer binding
- Allows the detection of many targets in a single reaction
CONVENTIONAL PCR
DPO™ PCR
Non-specific priming
Dual-specific priming
Decreased Specificity
No dimer formation – High Specificity
Decreased Sensitivity
High sensitivity
Difficulty in optimization
Tolerant to annealing temperature variation
Limitations in multiplexing
Reliable Multiple Target Amplification
TOCE™ technology allows for the detection and differentiation of many targets in a single fluorescence channel using catcher-melting temperature analysis (CMTA). The key components for TOCE technology are a DPO primer (providing high specificity), a pitcher (tagging oligonucleotide which hybridizes specifically to the target region) and a catcher (dual-labeled artificial template). Up to 10 targets can be detected with five fluorescence channels in a single reaction (5 channels) with the same sensitivity as single-plex real-time PCR. TOCE™ provides accurate and reliable results under various assay conditions, when compared to the capability of conventional real-time PCR that requires more reactions to achieve high multiplex testing.
- High multiplex in a single channel
- Up to 5 channels per reaction
- Multiple quality check steps for accuracy
- Specificity is highly maintained across a wide range of annealing temperatures and in the presence of a high concentration of non-target DNA
MuDT™, multiple detection temperatures, enables determination of individual Ct values for multiple targets in a single detection channel. With MuDT™, a standard five channel system can detect up to 15 targets and their associated Ct values supporting both high multiplexing and quantification without additional melting curve analysis. MuDT™ technology combined with DPO™ and TOCE™ differ from conventional PCR where only a single Ct value is determined in each channel.
- Multiple Ct (threshold cycle) values in each fluorescence channel
- Simultaneous detection, discrimination, and quantification of multiple targets in each channel of a single reaction
- Each Ct value of multiple targets present in a sample is equivalent to that obtained by single target amplification
- SNP genotyping in a single channel
Novaplex™ Assays are For Research Use Only. Not for Use in Diagnostic Procedures.
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Novaplex™ Assays are For Research Use Only. Not for Use in Diagnostic Procedures.
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Allplex™ 2019-nCoV Assay has an Emergency Use Authorization in the U.S.